Field Applications of a Traditional Laboratory Model System, the Mouse (Mus domesticus)
Jeffrey K. Wickliffe1, Brenda E. Rodgers1, Ronald K. Chesser1,3, Carleton J. Phillips1, Sergey Gaschak3, Igor Shohalevytch4, Igor Chizhevsky4, and Robert J. Baker1,2
1Texas Tech University, Lubbock, TX, U.S.A.
2Natural Science Research Laboratory, Lubbock, TX, U.S.A.
3International Radioecology Laboratory, Slavutych, UA
4Chornobyl Scientific and Technical Center for International Research, UA
Micronucleus Induction in Big Blue®
20,000 reticulocytes scored per individual (n=8)
Cells scored using the microflow™ flow cytometric assay
Laboratory model systems, Mus domesticus (3 strains), have been employed.
Fed standard laboratory feed ad libitum. Supplied with water ad libitum.
Radionuclides were internally deposited in measurable concentrations in all strains.
Exposure and dose for radiocesium estimated using whole body counts.
Sampled each individual twice (n=28)
Day 0-Preintroduction (reference)
Day 39-subchronic exposure
Assessed mitochondrial DNA heteroplasmy in 400 base pairs of the cytochrome b gene. 50 gene copies per individual.
No mutations observed in 80,000 base pairs examined to date (n=4 [n=2 subchronically exposed])
lacI Transgene Analysis
Following recommended protocol for blue plaque screen.
Uptake curves demonstrating the internal deposition of radiocesium (134,137Cs) in enclosed Mus.
Dose reported in milliGray (mGy)
Minisatellite Mutation Rates
Southern analysis of families conceived in enclosures
U. S. Department of Energy, U.S.A.
International Radioecology Laboratory, UA
Ministry of Emergency Situations, UA
Texas GAP Analysis Program, Wildlife and Fisheries Coop Unit, U.S.A.